Coding
ADH

Part:BBa_M36200:Design

Designed by: Kevin Aliado, Steven Lee, Simon Zheng   Group: Stanford BIOE44 - S11   (2011-12-04)


ADH; converts ethanol to/from acetaldehyde; no STOP codon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 892
    Illegal PstI site found at 991
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 892
    Illegal PstI site found at 991
    Illegal NotI site found at 1124
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 867
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 892
    Illegal PstI site found at 991
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 892
    Illegal PstI site found at 991
    Illegal AgeI site found at 630
    Illegal AgeI site found at 664
    Illegal AgeI site found at 855
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 520


Design Notes

The construct is codon optimized for Escherichia coli.


Source

This construct originally codes for Alcohol Dehydrogenase in Acetobacter pasteurianus. Amino acid sequence for Acetobacter pasteurianus can be found here: http://www.ncbi.nlm.nih.gov/nuccore/NC_013211.1?from=15149

References